Sakthivel, DSrinivasan, MRKarthik Venkatesh, PTANUVAS2021-12-072021-12-072021https://krishikosh.egranth.ac.in/handle/1/5810178744TNV_PIJ_2021_10(7)1211-1214Zebrafish (Danio rerio) is an emerging alternative animal model used for screening pharmacological effects and safety studies. One of the bottlenecks for the lack of PK data in zebrafish is the inability to collect the volume of blood required for bioanalysis. To make zebrafish a suitable alternative animal model to mammalian species to study the PK profile of a drug, we have used the skeletal muscles as an alternative to blood for bioanalysis based on the assumption that they may exhibit an equilibrium with the concentration of drug in blood and it is easy to collect muscle after euthanasia. Hence the current study was undertaken to assess the comparative pharmacokinetic profile of enrofloxacin administered by oral route, intraperitoneal (IP) route, and mixed in aquarium water. The fishes were euthanized and the skeletal muscle was collected at various time points. An assay of Enrofloxacin in skeletal muscle was done using a validated HPLC method. The AUC (μg/g.h) values for oral, intraperitoneal and exposed water routes are 2.82, 2.91 and 4.34 respectively, Cmax (μg/g) values are 0.71, 0.64 and 0.30 respectively. The results revealed that the oral and intraperitoneal (IP) route showed a better PK profile than mixed in water for enrofloxacin. The results show that the PK of enrofloxacin administered by oral and intraperitoneal route showed the typical absorption and elimination phase, whereas enrofloxacin mixed in water did not show the absorption and elimination phase. Hence it is reasonable to conclude that zebrafish can be used as an alternative model to mammals for understanding the PK of a drug. However further studies are required to ascertain their utility as an alternative to studying PK or TK of drugs.EnglishVeterinary ScienceArticle