NATH, A.K.THAKUR, AAYUSHEE2018-03-142018-03-142018-02http://krishikosh.egranth.ac.in/handle/1/5810041764ABSTRACT The present investigation was carried out to develop an efficient micropropagation system for runner tips of virus tested strawberry cv. Sweet Charlie. The runner tips were sterilized with HgCl2 for 2 minutes and established in MS medium supplemented with 0.5 mg/l BAP. Highest response of shoot multiplication was obtained in MS medium containing 0.50 mg/l BAP, 1.00 mg/l GA3 and 0.30 mg/l IBA. The multiplied shoots were rooted in MS basal medium supplemented with different concentrations of IBA. The highest average root length and average number of roots were produced in MS medium containing 0.50 mg/l IBA. The plantlets, thus developed were hardened and successfully established in a mixture of sand, soil and cocopeat in the ratio 1:1:1.In order to obtain true to type plantlets with the aid of tissue culture, it was quite necessary to examine the genetic uniformity of in vitro raised plants. Hence, in conclusion the two kinds of markers that is RAPD and ISSR markers can be successfully used to determine the genetic uniformity of in vitro raised plants. The protocol thus developed for micropropagation of strawberry cv. Sweet Charlie can be used over a long period of time without risk of somaclonal variations.ennullThesis