B. M. RAVINDRANATHSHAISTA FIRDOSE2024-01-032024-01-032022https://krishikosh.egranth.ac.in/handle/1/5810205587The present study was carried out to compare the hematobiochemical profile and serum antioxidant enzyme status of TVT affected and healthy dogs. Dogs presented to the clinic of Department of Veterinary Gynecology and Obstetrics were selected for the present study. Two groups of dogs were included in the study. Group I comprised of healthy dogs (n=12) and Group II (n=12) involved TVT affected dogs. Based on clinical signs, nature of lesions, impression smear cytology and histopathology, TVT was confirmed in 12 dogs. Blood samples were collected from both the group of dogs for hematobiochemical parameters and antioxidants evaluation. Quantification of the antioxidant enzyme activity was done using a microplate reader. In the present study, male dogs and dogs belonging to the age group of 2-4 years were the most affected. Among the various breeds of dogs, highest occurrence was found in Labradors. Clinical signs included hemorrhagic discharge from the external genitalia, licking of genitalia and foul odour. A significant leukocytosis with neutrophilia, significantly higher ALT, superoxide dismutase (SOD), glutathione peroxidase (GPx) and significantly lower catalase (CAT) activity were the prominent findings in haematobiochemical profile of dogs with TVT. Histopathology revealed tumor cells arranged in confluent sheets or rows within the stroma of thin fibrous connective tissue. Frequent mitotic structures were seen in the TVT cells along with infiltration of inflammatory cells in the connective tissue stroma. Further studies involving essentially large sample size may throw better light to draw more conclusive inferences by evaluating other oxidative stress markers and antioxidant parameters in dogs with TVT.EnglishThesis