Kamdi, B. P.Erra Srikanth2024-11-042024-11-042024-04-10https://krishikosh.egranth.ac.in/handle/1/5810216009Respiratory mycoplasmosis, is a very severe disease among all Mycoplasma infections in goats. It shows high mortality and morbidity rates in the goat population, ultimately responsible for substantial economic losses to the farmers. This study aimed to describe pathomorphology and molecular characterization of Mycoplasma spp. causing respiratory mycoplasmosis in caprines. Out of 790 screened animals, total of 155 pneumonic lung tissue samples were collected from the vicinity of Akola, Maharashtra. All the samples were subjected to DNA extraction followed by PCR, using Mycoplasma genus specific primers. Out of 155 samples 54 (34.8%) samples were positive for genus Mycoplasma with specific amplicon size of 280 bp by PCR. Subsequently, Mycoplasma genus positive samples were subjected to M. ovipneumoniae and Mccp identification by using species specific primers. Among 155 caprine lung samples, 39 (25.16%), 15 (9.6%) and 6 (3.87%) samples were positive for M. ovipneumoniae, Mccp and concurrent infection, respectively. Phylogenetic analysis of Mycoplasma isolates from the current study confirmed the isolates as M. ovipneumoniae and Mccp. Histopathological studies of Mycoplasma positive lung tissue samples revealed various types of pneumonia include, bronchopneumonia, interstitial pneumonia, bronchointerstitial pneumonia, with the most characteristic lesions such as fibrinous pleuritis, bronchitis with BALT hyperplasia, bronchial and bronchiolar epithelial hyperplasia, necrotic alveolitis with type II pneumocytic hyperplasia, thickened interalveolar and interstitial septae, syncytial cell formation, goblet cell hyperplasia, and marked infiltration of inflammatory exudate rich in neutrophils and mononuclear cells. Present study warrants to conduct large scale molecular studies for understanding the concept of patho-epidemiology of mycoplasmosis in goats to implement preventive strategies to minimize production losses in farmers.EnglishThesis