Sindhu, S. S.Shashi Bala2016-11-082016-11-082011http://krishikosh.egranth.ac.in/handle/1/84483The present study was conducted on the three cultivars of chrysanthemum (Mayur, Dolly Pink, Royal Purple). The objective of the study was to developed an efficient protocol for direct regeneration, multiplication and rooting in vitro conditions and checked the genetic fidelity of the micropropagated plants. Nodal segment and shoot tip explants, after being sterilized with 0.1% of mercuric chloride and 70% ethanol, were inoculated in Murashige and Skoog (MS) media with varied concentrations of indole acetic acid (IAA), benzyl amino purine (BAP), napthelic acetic acid (NAA)and their combinations. Different parameters including shoot regeneration percentage, multiplication average number of shoots per explant, length of shoots (cm), number of leaves per shoot, number of days taken for root initiation, number of roots per shoot, length of root (cm), were studied during the course of study. Intermediate concentrations of benzyladenine purine (BAP 1.0 mg/l) was found superior to all the other BAP concentration. MS media fertified with 1.0 mg/l BAP had produced the maximum shoots (90.53 per cent in shoot tip and 90.80 per cent in nodal segment), shoots per explant (4.06), length of shoot (3.90 cm), number of leaves (4.93). Similarly, when the combination of different concentration of IAA and BAP were used, significant results regarding the regeneration in chrysanthemum plantlets were achieved. Satisfactory rooting response was obtained with (2.0 mg/l and 1.5 mg/l IBA0. In vitro raised plantlets were transferred to potted soil for the hardening and after hardening the leaves were collected from the potted chrysanthemum plants for DNA extracts. The maximum survival was found (100%) in potting mixture sand +soil+FYM (1:1:1) and sand+soil+vermicompost (1:1:1). Twenty primers were used for DNA amplification. Out of twenty primers screened, thirteen primers produced amplification. Reproducible and clear banding patterns were obtained in a reaction mixture of 10μl containing 50 ng template DNA, 100 μM of dNTPs mix, 1 μM of primer, 1.5 Mm MgCl2 , 1μl of 10 X Taq DNA polymerase buffer and 2.0 units of Taq DNA polymerase. All the bands were similar and no polymorphism was to be found, which showed that all the plants raised through micro propagation were true to type or identical to the mother plants.enChrysanthemum, DNA amplification, BAP, IBA, MicropropagationThesis