Garg, S. R.Chauhan, Sneh Lata2017-12-132017-12-132017http://krishikosh.egranth.ac.in/handle/1/5810037473In the present study, High Performance Liquid Chromatography with Ultra-Violet detector (HPLC-UV) technique and with florescence detector (HPLC-FL) was standardized and validated for the detection and quantitation of tetracyclines (oxytetracycline, tetracycline and chlortetracycline) and aminoglycoside (kanamycin and gentamicin) in milk respectively. The validation procedure showed that the values for the system precision (% RSD for all analytes were less than 12.68% for area and 1.12 % for retention time). Linearity (R2>0.99), specificity and accuracy (70-110%) and precision (<10%) were within accepted range and demonstrated the system and method suitability for analysis of milk samples. The standardized and validated method was used to detect tetracycline and aminoglycoside residues in 100 milk samples collected randomly from local market of Hisar (Haryana). A total of 33 samples were found to be containing antimicrobial residues, out of which 18 samples were positive for tetracycline residues and 15 samples for aminoglycoside residues. Maximum positive samples were associated with vendor milk followed by dairy and pasteurized milk. Mean concentrations of oxytetracycline, tetracycline and chlortetracycline antimicrobial residues in market milk samples were 12.45, 1.11 and 7.69 μg/kg, respectively, while that of kanamycin and gentamicin were 1.27 and 65.06 μg/kg, respectively. Comparison of antimicrobial concentration in each positive sample of milk with international MRLs showed that gentamicin and oxytetracycline were responsible for maximum violations. The risk assessment based on per capita availability of milk in Haryana and India by calculating hazard quotient for each analyte under study revealed no risk due to dietary exposure to tetracycline and aminoglycoside residues through milk.enThesis