Vijayakumar, N KSandeep SharmaKAU2019-05-232019-05-232000171698http://krishikosh.egranth.ac.in/handle/1/5810104375PGThe study under the title 'Micropropagation of teak (Tectona grandis Linn.) through in vitro techniques' was carried out at College of Forestry Vellanikkara during year 1998-2000. The objective of the programme is to standardize the method of clonal propagation of teak through teak tissue culture technique. The explants used were from one to two year old seedlings Culture contamination mainly due to fungus was prominent in the ramy season. Prophylactic spraying of a mixture of fungicide (Bavistin and Indofil M-45 (0.1% each) on source material followed by fungicide dip to explants in similar solution for 30 min and surface sterilization treatment in 0.15 per cent mercuric chloride for 15 min controlled the contamination. Phenol exudation was contained effectively by supplementation of citric acid and ascorbic acid (150 mgl' each), to the media. It was also in low quantity when explants of smallest size (1 cm long below the node) were used. Murashige and Skoog (MS) medium was found to be better than 12 MS and WPM. Individual supplementation of BA to MS media was found effective than other cytokinins. However, MS media supplemented with Kinetin and !AA 0.5mgrl each was found to be the best media for shoot proliferation. A maximum of2.20 and 2.11 number of shoot shoots per explant could be induced in MS + 0.5 mg l' BA and MS + 1.0 mg l' BA + 0.1 mg rl!AA + 1.0 mg I-I 2ip respectively. Addition of growth supplements like coconut water, activated charcoal, adenine sulphate, and casein hydrolysate did not have any favourable effect on growth and establishment. Maximum in vitro rooting (56.25%) was obtained on Yz MS + 0.4 mg r' NAA + 4.0 mg rl !AA +0.25 per cent AC with pulse treatment (dip to excised shoot base in 1000 mg r' !AA solution for 2 min) and after transferring to auxin free media after 7 days. Under ex vitro rooting trials the maximum percentage of rooting (87.50) with highest root length (3.0 cm) was obtained on vermiculite. Sand and vemiciilite were found to be the best media for hardening of in vitro raised plantlets. The hardened plants were transplanted to polybags containing ordinary potting mixture and treated like a normal conditioned seedling. Hardened seedlings were out planted in the field. The technique developed for the micropropagation of teak during present investigation can be used for large scale clonal multiplication of the species.ennullThesis