Rajesh KumarBisht, Ritika2019-11-042019-11-042019-08http://krishikosh.egranth.ac.in/handle/1/5810133992Fowl adenoviruses (FAdV) are the ubiquitous and cause extensive damage to the poultry industry. They are known to be associated with various disease conditions such as Inclusion body hepatitis, Hydropericardium Syndrome, respiratory disease, tenosynovitis, impaired growth, reduced egg production, aplastic anemia, atrophy of bursa and thymus, enteritis and conjunctivitis in chickens and other birds. Present study was undertaken to study epidemiology, to isolate and perform molecular analysis of fowl adenoviruses from the chickens with the respiratory disease conditions. Natural outbreaks investigated during the study revealed 2 –15% mortality among the affected birds aged 3-6 weeks. General postmortem findings were enlarged and friable liver with necrotic foci, enlargement of kidney, congestion and haemorrhagic trachea, congested lungs. Four isolates of fowl adenoviruses were propagated in primary CEL cell culture. The cytopathic effects were characterized by rounding of the cells, degeneration, increase in the refractive index of the cells and microplaques formation. AGPT, CIEP, IFAT, Dot-ELISA and Sandwich ELISA determined the presence of the virus in infected tissues and cell culture. Intensely basophilic intra nuclear inclusion bodies were also visualized following MGG staining of CEL cells. The genomic DNAs were extracted from the infected CEL cells and PCR was carried out for amplification of L1 region of the hexon gene showing amplicons of the ~900 bp. On digestion with MluI, three isolates were digested and the two bands of 400 and 500 bp were visualized and with the enzyme StyI, only one isolate was digested and two bands of 480 and 420 bp were obtained. None of the isolates were digested by the enzymes BglI, BsiWiI and ScaI. RE analysis revealed that isolates belong to two serotypes 5 (R-66) and 8 (R-53, R-43, R-63). Thus, it can be concluded that these serotypes are involved in respiratory disease of domestic chickens in India.ennullThesis