BRAHMAPRAKASH, G. P.LAVANYA, G.2017-06-282017-06-282010-07-10Th-9676http://krishikosh.egranth.ac.in/handle/1/5810023616The present study focused on developing a protocol, separation of compounds by chromatographic technique and screening antimicrobial compounds from selected medicinal and aromatic plants. Antimicrobial compounds from two medicinal (Lantana camara and Chromolaena odorata) and two aromatic (Pogostemon patchouli and Rosmarinus officinalis) plants were screened for their antimicrobial activity against four bacteria (Escherichia coli, Staphylococcus aureus, Xanthomonas campestris and Erwinia sp.) and two test fungi (Candida albicans and Fusarium oxysporum). Plants were extracted with different polar (ethanol and ether) and non-polar (chloroform and hexane) solvents. TLC profiling of all the plant extracts in different solvent system indicated the presence of different groups of phytochemicals in these plants. Terpenes, phenolic acids and flavonoids were found to be present in the biomass extracts of the plants used and the Rf values ranged between 0.3 to 0.95 for these compounds. Terpenes, phenolic acids and flavonoid compounds were effective in inhibiting the growth of microorganisms. Flavonoids from Lantana camara (Lantana) of ethanol extract were highly active against Fusarium oxysporum and phenolic acid group of compounds from chloroform extract of Rosmarinus officinalis (Rosemary) highly inhibited Xanthomonas campestris. Compounds from plant extracts showed highest activity against plant pathogens tested. Ethanol solvent was found to be better solvent for purifying the compounds followed by the solvent chloroform and ether. Hexane solvent was found ineffective in dissolving and separation of compounds.ennullThesis