Koti, R.V.Akshay, N.2024-07-182024-07-182018-06https://krishikosh.egranth.ac.in/handle/1/5810212209A field experiment was carried out during kharif-2017 at Main Agriculture Research Station, University of Agricultural Sciences, Dharwad to screen the F2 population obtained from the two crosses TMV 2 × ICGV 86031 (TIP) and JL 24 × ICGV 86031 (JIP) for lime induced iron chlorosis (LIIC) resistance. Variation was observed among the selected population of F2 generation with respect to visual chlorotic rating (VCR) score at 45 days after sowing (DAS) and relative chlorophyll content at 60 DAS. The iron deficiency chlorosis (IDC) resistant parent ICGV 86031 had significantly higher relative chlorophyll content (46.2) compared to IDC susceptible parents TMV 2 (39.3) and JL 24 (37.7), respectively. Similarly, ICGV 86031 showed least VCR score (1.00) compared to TMV 2 (2.10) and JL 24 (2.30). Among the progenies, TIP-16 and JIP-27 had recorded highest relative chlorophyll content (41.8 and 42.9, respectively) with least VCR score (1.07 and 1.00, respectively) followed by the populations TIP-23 (1.15) and JIP-28 (1.00). The hydroponic experiment was conducted, to unearth the strategy I mechanism involving rhizosphere acidification and root ferric reductase activity in parents of the crosses, ICGV 86031, TMV 2, JL 24 and check KADIRI 9. The results on physiological and biochemical observations indicated that ICGV 86031 at 45 days recorded highest leaf ferrous content (7.44 ppm), chlorophyll content (2.41 mg g-1 fresh weight), relative chlorophyll content (42.60), root ferric reductase activity (56.9 n mol g-1 fresh weight hr-1) and root protonation (pH 3.83), followed by KADIRI 9 and significantly least leaf ferrous content (4.61 ppm), chlorophyll content (1.24 mg g-1 fresh weight), relative chlorophyll content (31.48), root ferric reductase activity (40 n mol g-1fresh weight hr-1) and root protonation (pH 4.32) was observed in JL 24 followed by TMV 2. Hence, the parent genotype ICGV 86031 has been identified as most resistant for LIIC.EnglishThesis