K. G. NarayanMehta Ranajee2023-11-172023-11-171989https://krishikosh.egranth.ac.in/handle/1/5810201379A total of 39 stock cultures of S. perfringsns were obtained. Sixteen of these cultures included twelve from England and Wales, one from America, two from All India Institute of Medical Sciences, New Delhi and one from Haryana Agricultural University, Hissar. The rest twenty three cultures were isolated in Ranchi. All were earlier studied and maintained in the Department of Veterinary Public Health and Epidemiology. These cultures were studied for their morphological and biochemical characters for re identification. All were grown in sporulation medium for two different periods viz 10 and 25 hours to harvest cell free supernatant of frozen thawed cyclomixed culture, while all the 156 (39 strains x 2 growth period * 2 preparations) materials were tested for haemagglutinin active on sheep and human erythrocytes, 78 (39 strains x 2 growth periods viz cell free supernatant of frozen-thawed cyclomixed) were tested for enterotoxin by microplate ELISA method. DOZ-immune binding technique viz DOT-ELISA for the assay of enterotoxin was developed. The morphological and biochemical characterization of the 39 test strains revealed that 21 of the 23 Ranchi-strains were raffinose-non fermentor. This raffinose-negative cultures constituted a cluster unique to Ranchi. Haemagglutinin production was not found to common. Haenagglutinin active on human erythrocytes appeared to be more common than those acting on sheep erythrocytes. Nineteen out of thirty nine strains were active on human erythrocytes, while only eleven strains were so on sheep erythrocytes.EnglishThesis