Dr Sanjay ShakyaVIVEK KUMAR NAIK2024-08-292024-08-292024https://krishikosh.egranth.ac.in/handle/1/5810214018The present study was undertaken to explore the zoonotic infections due to Mycobacteria spp., Brucella spp., and Coxiella burnetii. The research aimed to unravel the transmission dynamics of these pathogens between small ruminants and in-contact humans, employing molecular detection methods such as Multiplex PCR for Mycobacteria bovis detection, PCR for Brucella genus identification, AMOS PCR for Brucella species identification, and real-time PCR for Coxiella burnetii detection. The study utilized specific genetic markers, including RD4 and RD1, B4 and B5, AMOS with one reverse primer, and Com1 primer for Mycobacteria spp., Brucella spp., and Coxiella burnetii detection, respectively. Risk factor analysis was conducted using a questionnaire method and SPSS software for univariable and multivariable logistic regression. The study unveiled a 0.75% prevalence of Mycobacteria bovis in small ruminants, with a corresponding herd-level prevalence of 2.6%. Notably, individual animallevel risk factors, encompassing species, age, and sample type, failed to demonstrate statistical significance. Conversely, herd-level risk factors, specifically the number of small ruminants (OR = 1.158, p = 0.003) and the introduction of new animals (OR = 1.090, p = 0.034), were found to be significant. Multivariable logistic regression further revealed that a larger flock size (OR= 1.15, p= 0.004) was significantly associated, emphasizing the complex nature of zoonotic tuberculosis transmission and underscoring the imperative for continuous research to facilitate effective control measures. For Brucella spp., the study disclosed 2.26% prevalence of Brucella melitensis in small ruminants, with variations in species, gender, age, and flock size. Among the 131 small ruminant flocks, 14 flocks were found positiveEnglishThesis