Sangwan, M.L.Bhuyan, Dhiraj Kumar2017-08-052017-08-052009http://krishikosh.egranth.ac.in/handle/1/5810027513The present study was conducted on Murrah buffaloes including 35 progeny and mother pairs and 11 bulls to develope DNA finger printing technique and to estimate the genetic diversity among populations using six microsatellite loci. The genomic DNA from the blood was isolated using Sambrook and Russel method (2001) with slight modification and from semen using Aravindakshan (1998) with slight modification. The yield per sample was ranged from 200 to 300 μg which is diluted to approximately 30 ng per μl for the present study. The total number of alleles for all the 6 loci ranged from 3 (ETH152) to 11 (CSSM61) with the mean number of alleles was 6.33 per locus. The observed heterozygosity values across the population was lowest for the locus CSSM43 (0.375) and highest for the locus CSSM61 (0.775). The mean observed heterozygosity and mean expected heterozygosity was found 0.5439 and 0.6209 respectively. The mean PIC value for all loci was 0.5661. In the present study, 5 microsatellite loci were found to be highly informative. The results revealed two deviations from the HWE in the populations for the loci ILSTLS28 and CSSM22. The mean FIS for all loci was 0.1266. Marker at loci ILSTS17 was not neutral. The combined exclusion probability for all loci was found to be 0.845. The investigated set of 6 microsatellites can be successfully used for parentage testing purposes in Murrah buffalo.enThesis