SHARMA, RAJNISHGARIMA2021-12-022021-12-022021-11https://krishikosh.egranth.ac.in/handle/1/5810178505ABSTRACT The present study was aimed to assess genetic diversity based on PCR-based molecular markers in an endangered ethno medicinal herb of Himalayan region, Nag chhatri (Trillium govanianum Wall.ex D.Don). Total 38 genotypes were procured from high altitude regions comprising of four districts viz., Kullu, Shimla, Chamba and Sirmaur of Himachal Pradesh and were characterized based on ISSR and SSR marker analyses. Based on obtained DNA profiles as well as factorial and neighbour joining tree analyses, minimal genetic diversity was observed using both the markers. On an average, the genotypes from Kullu and Chamba regions depicted to be more diversified compared to each other with respect to the other two locations taken into consideration. The admixture of two genetic pools (K=2) was observed among the subjected genotypes using structure programme version 2.3.4. The low levels of genetic diversity could be due to the immense population decrease, vegetative proliferation, presence of meiotic irregularities towards pollen sterility, poor seed viability, inbreeding depression, limited seed dispersal, slow life cycle and specific habitat requirement, etc. Therefore, the present findings are emphasizing on executing efficient prioritized effective means (ex-situ propagation measures and strict in-situ conservation policies) towards its biodiversity conservation due to limited gene flow among the populations in natural habitatEnglishThesis