Warke, S. R.Koul Viraj2024-01-312024-01-312023-03-28https://krishikosh.egranth.ac.in/handle/1/5810206622Group A rotavirus (RVA) is one of the major pathogens causing gastroenteritis in humans and animals worldwide. The virus contains 11 dsRNA segments encoding six viral structural proteins (VP) and six non-structural proteins (NSP). It causes moderate to severe gastroenteritis in neonates of all species. In the present study, 50 faecal samples each were collected from pups, piglets and human infants showing clinical signs of diarrhoea from Nagpur region. Antigen detection kit revealed the overall prevalence of rotavirus infection in canines as 6% (3/50), piglets 12% (6/50) and human infants 8% (4/50), respectively. The positive samples were tested for rotavirus group A, using onestep RT-PCR specific to the VP6, VP4 and VP7. All 13 samples were found positive for VP6 gene, while three samples from human infant and one from piglet showed VP4 and VP7 genes. VP4 and VP7 gene could not be detected from any canine samples. One representative amplicon of VP6 gene, each from canine, piglets and human infants and one amplicon each of VP4 and VP7 from piglets and human infants were sequenced and subjected to phylogenetic analysis. VP6 gene from human infant (H19) showcased high diversity amongst the compared sequences and may be showing defined diversity among tested sequences. Sequence from piglet (P-16) was 93.80 % homologous with other sequence from pig of Maharashtra region (Accession No. LC379954.1). The sequence from canine (C- 8) was 100% identical with a sequence from China (Accession No. OL388440.1). VP7 gene sequences from human infant (H-19) and piglet (P-16) were distantly located from each other and placed under different clusters. VP7 sequence from H-19 had 97.33 % homology with a sequence from human sample of Maharashtra (Accession no. LC377480.1). VP7 sequence of P-16 had 100 % homology with that from a pig from Maharashtra (Accession No. LC377485.1). VP4 sequence from human infant (H-19), was placed under the same cluster as that of pig (P-16). The amplified sequences showed interspecies cross relationship with more than 90 % homology. This suggests that there is not much variation among the sequences of VP4 gene of rotavirus amplified from human infant and pig in this study. The sequence of VP4 obtained from H-19 had 97.47% homology to a sequence of human sample from Maharashtra. Similarly, the sequence of P-16 had 93.80% homology to a sequence from pig from Maharashtra.EnglishThesis