PHUKAN, A.Gupta, Santosh Kumar2019-08-032019-08-032017-07http://krishikosh.egranth.ac.in/handle/1/5810118906The present investigation was carried out with the view to study the anthelmintic activity of Entada phaseoloides and Erigeron linifolius plant extract against gastrointestinal helminth of goat. The overall prevalence of gastrointestinal nematodes in goats was found to be 82.75 per cent under the prevailing agro-climatic condition of Byrnihut during the period of October to November 2016. The different nematodes recorded in goats were Haemonchus spp. (82.75%), Oesophagostomum spp. (78.16%), Trichostrongylus spp. (70.11%), Strongyloides spp. (62.06%), Bunostomum spp. (48.27%), Trichuris spp. (39.08%) and Cooperia spp. (24.13%). The EPG count in treated groups (plant extracts /drug) decreased gradually with commencement of treatment. In Group A and B treated with crude methanolic extract of Entada phaseoloides and crude ethanolic extract of Erigeron linifolius, EPG became nil on 21st day. In group C treated with combination of both plant extracts, EPG gradually reduced but was not nil on 21st day. While group D treated with fenbendzole, EPG count became nil on 7th day of post treatment. Group E was kept as untreated infected group, where the EPG count gradually increased and Group F, the animals where kept as healthy control. During pre-treatment period, the haemato-biochemical study revealed a significant decrease in the levels of Hb (7.23 ± 0.13), PCV (25.75 ± 0.48), TEC (9.77 ± 0.17), MCV (21.73 ± 0.53), MCH (7.11 ± 0.16), Lymphocytes (42.75 ± 0.50), basophils (0.25 ± 0.04) , total serum protein (5.20 ± 0.18), serum albumin (2.34 ± 0.05), serum globulin (2.84 ± 0.19), A:G, serum sodium (127.37 ± 1.51) ,serum chloride (90.93 ± 1.00), serum potassium (3.18 ± 0.28), serum glucose (43.89 ± 0.80) , serum iron (110.41 ± 2.05) and serum zinc level (51.72 ± 1.35) in the affected goats which increased gradually after treatment whereas an increase in the levels of TLC (15.75 ± 0.93), eosinophils (3.86 ± 0.14) Monocytes (1.71 ± 0.14), and neutrophils (45.78 ± 1.37) were observed in the affected goats which also decreased gradually after treatment and reached normal level. Based on reduction of EPG count and haemato-biochemical changes, the efficacy of methanolic extract of Entada phaseoloides was 72.52, 89.00 and 100 % followed by ethanolic extract of Erigeron linifolius was 62.76, 86.17 and 100 % while in combination was 53.68, 73.68 and 89.47 % on 7th , 14th and 21st day respectively. Fenbendazole showed highest efficacy 100% on 7th day post treatment. In-vitro studies were undertaken for which adult parasites were collected from local slaughter house. Parasites were exposed to different concentration of plant extract i.e. 50, 150, 300, 1000 and 3000 µg/ml PBS, petri dish containing fenbendazole was kept as positive control while in negative control parasite were exposed to only PBS. Both plants extract either individually or in combination were effective against adult parasite of Paramphistome cervi, Haemonchus contortus and Fasciola gigantica. Further it was revealed that dose dependent anthelminthic activity was exhibited by plant extract and highest efficacy was observed at 3000 µg/ml.ennullThesis