KAUR, MOHINDERTHAKUR, DEEPSHIKHA2017-01-192017-01-19201047135http://krishikosh.egranth.ac.in/handle/1/97027ABSTRACT Under the present study, an attempt was made to isolate and characterize the Phosphate solubilizing strains of Pseudomonas sp. from the rhizosphere of normal and replant sites of apple and pear from different locations of tehsil Nankhari, district Shimla. The aim of the study was to select and develop best PGPR strain of fluorescent Pseudomonas sp. for management of replant problem. The thirty Pseudomonas isolates were screened out for phosphate solubilizing potential along with other plant growth promoting activities such as siderophore production, antifungal activity, proteolytic activity, HCN and ammonia production, production of plant growth regulators i.e. auxins, gibberellins and cytokinins. Out of thirty isolates five isolates were selected for further characterization and optimization of cultural conditions for mass multiplication of microbial biomass and preparation of inoculums for field trials. The best levels of these factors were NBRI-P, 48 h of incubation time, 28 ºC temperature and pH 7.0.enThesis