Kulkarni, M SRama Krishna, M V S2019-08-212019-08-212000No. of references 120http://krishikosh.egranth.ac.in/handle/1/5810122861"Jasmine chlorotic spot virus (JCSV) disease, is present in almost all fields of J. sambac of North Karnataka, and causing considerable damage. The incidence ranged from 3.00 to 95.00 per cent. The JCSV caused chlorosis of the plant, chlorotic spots and malformations of leaves on J. sambac whereas symptoms were different viz., chlorosis extending along the veins sometimes with rough circular shape on J. multiflorum. The other two species J. auriculatum and J. grandiflorum never exhibited symptoms indicating their resistance. The leaf samples of both J. sambac and J. multiflorum showed negative reaction with TSWV, CMV and PVY tested through ELISA. The electron microscopic observations with infected J. sambac leaves revealed presence of elongated flexuous particles of approximately 630 nm size. These particles were not reacting with any of twelve antisera of other known viruses belonging to Poty, Potex, Caria and Clostero virus groups in ISEM test. Ultrathin microtomy revealed the presence of some laminated rings of virus inclusion bodies in leaf sample sections from J. multiflorum only. The Jasmine chlorotic spot causing virus was not sap transmissible, also not transmissible by insects and soil. But, this was transmissible through approach grafting and through vegetative cuttings using infected material in the nurseries. Based on Symptoms, Electron microscopy, Serology, Transmission studies and Host range the virus associated with the Jasmine chlorotic spot disease in Karnataka has been concluded to be a probable member or strain of CarIa virus group. Hot water treatment was not effective for management of JCSV of J. sambac as the temperatures necessary for therapy had deleterious effect on survival of vegetative cuttings. Dipping cuttings in leaf extracts of coconut, maize and mirabilis (5%) showed promising results in inhibiting the JCSV infecting both J. sambac and J. multiflorum."ennullThesis