Mallesha, B.C.Archana, K.2023-01-032023-01-032021-12-31Th-13085https://krishikosh.egranth.ac.in/handle/1/5810191024Yeasts were isolated from leaves and rhizosphere of Red gram, Green gram, Black gram, Cowpea and Bengal gram collected from Bengaluru and Dharwad Districts of Karnataka. Thirty-five yeast isolates obtained were biochemically characterized, Screened for their biocontrol and plant growth promotion activities. Yeast isolate, RG19SI28 (Pichia fermentans) showed the highest per cent inhibition of usarium oxysporum (41.37) and Sclerotium rolfsii (35.81) and highest phosphate solubilization index of 5.73. Greenhouse evaluation of two efficient yeast isolates for plant growth promotion in green gram crop was studied in two seasons. The highest germination percentage of green gram was recorded by the inoculation of yeast isolates RG19SI28 (98.33) and GG7SI9 (96.67). Based on the partial sequence of 18S rRNA, the efficient yeast isolates were identified as Pichia fermentans (RG19SI28) and Kluyveromyces marxianus (GG7SI9) from the NCBI database through BLAST analysis. Foliar application (unsterilized soil) method of inoculation found to increase plant growth (plant height, root length and number of nodules) and yield (number of pods and seed yield) parameters in both the seasons compared to seed treatment method of inoculation. K. marxianus performed better in improving green gram plant biomass and yield in both the seasons followed by P. fermentans along with the bio-inoculants and fertilizer applicationEnglishThesis