Kang, S. S.Sharma, Shikha2016-09-182016-09-182014http://krishikosh.egranth.ac.in/handle/1/77284Cucurbits are known to be affected by many viruses, among them aphid transmitted +ssRNA viruses viz. potyviruses and cucumoviruses of family potyviridae and bromoviridae, respectively are considered most destructive viruses in terms of severe yield loss, causing mosaic, blistering kind of symptoms. In the present study detection of Cucumber mosaic virus and Zucchini yellow mosaic virus was done from major cucurbits grown at vegetable research farm PAU, Ludhiana. A total of 54 genotypes were screened under natural and artificial conditions. Typical mild to severe mosaic, pronounced blistering kind of symptoms were observed in susceptible check ‘Punjab Sunehari’ after one week of sap inoculation. Three lines viz. MM 4021, SM MC 20, MM 4078 were found resistant against CMV and ZYMV infection. Resistant lines were confirmed for absence of virus titre using DAS-ELISA. Variable symptoms resembling leaf deformation accompanied with yellowing and mild blistering were found in mixed infection of Tomato leaf curl virus New Delhi strain with ZYMV from bittergourd. Cucumber mosaic virus subgroup IB, Zucchini yellow mosaic virus, Papaya ring spot virus-P were characterized from cucurbits in Punjab. Role of potato is confirmed in harbouring all the viruses associated with cucurbits viz. CMV subgroup IB, ZYMV as well as Papaya ring spot virus-P. Aphids associated with cucurbits and potato were identified as belonging to species Aphis gosypii and Myzus persicae. These findings confirmed the role of survival of CMV and ZYMV through potato and possible transmission of both the viruses from one crop to the other during the harvesting of potato and sowing of cucurbits in Punjab.encucurbitsZYMVCucumber Mosaic VirusCMVZucchini Yellow Mosaic VirusThesis