Kikon, Yanben M.Loganathasamy, KGomathy, VSVijayarani, KTANUVAS2020-07-302020-07-302020-07http://krishikosh.egranth.ac.in/handle/1/5810150126TNV_IJCMAS_2020_9(7)3572-3579The present study was conducted to assess the sperm morphology and DNA integrity of frozen thawed buffalo semen treated with heparin binding protein (HBP). Buffalo semen straws from 10 bulls were procured from Central Frozen Semen Production and Training Institute, Hesseraghatta, Banglore-560088. The frozen straws were thawed at 37ºC for 30 seconds and emptied into a 15mL sterile plastic centrifuge tube containing 1mL capacitation medium (control), addition of 25μg/mL (treatment I), 50μg/mL (treatment II) and 100μg/mL (treatment III) of HBP. The contents were incubated at 37ºC for 2 hours. After incubation, the sperm morphology was determined by Rose Bengal stain technique. Morphologically normal sperm in control, HBP treatment I, II and III were 86.70% ± 1.52, 87.00% ± 1.21, 87.00% ± 1.28 and 86.55% ± 1.59 respectively. There was no significant difference between control and HBP treatments with respect to sperm morphology. The sperm DNA integrity was assessed by acridine orange stain method. There was no significant difference among control (90.15% ± 1.14), HBP treatment I (90.25% ± 1.25), II (89.85% ± 1.05) and III (90.40% ± 1.16) with respect to sperm DNA integrity. This study indicated that HBP supplementation in capacitation medium did not alter the sperm morphology and DNA integrity.enVeterinary ScienceArticle