Dr. C. J. DaveJoshi Om B.2017-02-212017-02-212015http://krishikosh.egranth.ac.in/handle/1/5810001997The present study was carried out on a total of 150 straight-run day-old Cobb-400 broiler chicks which were procured from Shakti Broiler Breeders Pvt. Ltd., Sarsa, Anand, Gujarat to evaluate pathogenicity of the IBH-HPS virus inoculation. A 20 % W/V solution prepared by triturating the liver in Phosphate buffered saline (PBS) was injected intramuscularly @ 0.5 ml in broiler chicks. For the purpose of evaluating pathogenicity, chicks were randomly divided into 3 equal groups, comprising 50 chicks in each group. Group I was considered as Control Group, i.e., without administering any inoculum. Group II birds were inoculated with IBH inoculum @ 0.5 ml i/m on 14th day. Six birds from Group II were sacrificed on 1st, 3rd & 5th day post-inoculation (PI; i.e., after 14th day) as per guidelines provided by IAEC. On the very next day post-inoculation blood samples were collected for haematological and serum biochemical analysis from randomly selected six birds under each group. The birds were sacrificed for evaluation of gross lesions as well as histopathological changes associated with experimental IBH infection. Group III were kept for monitoring normal mortality (i.e., without any intervention). Tissue samples (viz., liver, spleen, heart and kidney) were collected in 10% neutral buffer formalin for histopathological examination. Liver saenveterinary science, pathologystudyThesis