Dr. SHRIKANT KULKARNIGANGADHAR KAPASE2021-03-122021-03-122019-08https://krishikosh.egranth.ac.in/handle/1/5810162285The present experiment was designed to study the biochemical, physico-chemical and proteomic profile of different skeletal muscles viz., Vastus lateralis (VL), Gluteo biceps femoris (GBF), Gluteo medius (GM), Longissimus thoracis et lumborum (LTL), Psoas major (PM) and Semitendinosus (ST) and also to assess impact of postmortem aging on these parameters in sheep. Muscles were evaluated for various biochemical parameters viz., myoglobin, % metmyoglobin, R value, protein extractability, collagen content and solubility, TBARS ; physico-chemical parameters viz., pH, WHC, drip loss, MFI, muscle fiber diameter, WBSF and colour indices in fresh samples (day 0), 5th, 10th and 15th day of postmortem period using standard procedures. The SDS-PAGE followed by MALDI TOF MS/MS technique was employed in proteomic study. There were significant (p<0.05) differences between the skeletal muscles in various biochemical and physico-chemical parameters in fresh muscle samples (day 0). The R value, total protein extractability, sarcoplasmic protein extractability, myofibrillar protein extractability, collagen content and solubility, TBARS, pH, WHC, MFI, colour indices (L*, a* and b*) increased significantly (p<0.05) whereas myoglobin content and WBSF values decreased significantly (p<0.05) as the postmortem period advanced. Proteomic studies revealed the protein degradation in muscles due to postmortem aging. The proteins identified by MALDI TOF MS/ MS are Adenylate kinase isoenzyme, Intracellular hyaluronan-binding protein, Lymphocyte antigen 86, Actin, Integrin alpha-6, and PDZ and LIM domain protein 3. The significant differences in biochemical, physico-chemical and proteomic profile during different postmortem period indicated that many biochemical and structural changes occur during postmortem period that convert muscle to meatEnglishThesis