Dr. Dinesh P.T.MANASA M.2024-01-242024-01-242022-12-29https://krishikosh.egranth.ac.in/handle/1/5810206551The study was conducted to assess the healing potential of decellularised tissue engineered tri-phasic composite bio-ceramic (test graft) in comparison with tri-phasic composite bio-ceramic (control graft) as bone graft substitutes in rat calvarial critical size defect models. Four-millimeter diameter defects were created and grafted with test and control graft on left and right parietal bones respectively in 18 adult male Wistar rats, under general anaesthesia. Healing was evaluated by radiography, histology and assessment activity of bone turnover markers. Grossly, the test graft material was incorporated with regenerated bone by 8 weeks and control took 12 weeks to achieve the same. Serial radiographic evaluation revealed that the grafts were in position throughout the healing period. The test graft had integrated with the host bone radiographically by eight weeks whereas control graft took twelve weeks for complete integration. In the present study it was observed that activity of ALP and ACP were haphazard throughout the observation period owing to the fact that stage of healing in test and control side was not in par. Histological examination revealed complete healing of the defect grafted with test bioceramic by 4 weeks without any signs of inflammation or fibrous proliferation. On contrary, defect grafted with control bio-ceramic was not completely regenerated even by 12 weeks but it was filled with scattered islands of woven bone. By 12th week test graft was completely replaced by new bone, showing 100 per cent new bone formation whereas on control side it was only 29.29 per cent. In all the stages of healing, test material had the highest per cent of new bone formation that filled the defect than control. Therefore, the present study has proved the successful use of test bioceramic and its advantages over the already available bone substitutes for the treatment of critical sized defects in rat calvaria.EnglishThesis