SATHYANARAYANA, B.N.KHALID, AKHUNDZADA2021-09-222021-09-222018-08-01Th-12015https://krishikosh.egranth.ac.in/handle/1/5810176033Citrus species are propagated by both sexual and asexual methods. The present study was conducted to investigate into the in vitro responses of lime and mandarin in particular reference to seed. Sterilants such as alcohol, sodium hypochlorite, mercuric chloride were used. However, repeated trials with modiefied procedures done over a period of around six months, aseptic cultureof leaf and nodal segments could not be obtained. In studies on identification of endophytic microorganisms, revealed the presence of Colletotrichum spp. Hence, seeds were used as explant. In lime, highest percentage of germination, increased shoot length, increased number of leaves and increased root length was obtained when seeds were cultured on MS media containing BAP at 3 mg/l and BAP + NAA at 5 + 0.5 mg/l. In mandarin media containing Kinetin at 5 mg/l and Kinetin + NAA at 3 + 0.1 mg/l, increased values of germination percentage and other growth parameters were obtained. Callusing from seeds during germination could be found in media containing BAP at 1 mg/l. Organogenesis could be induced when the calli were cultured on media containing Kinetin at 1 mg/l. When auxins were supplied at a higher concentrations above 1 mg/l irrespective of cytokinin content, a detrimental effect on seed germination and callus initiation was observed. When nodal, internodal and cotyledonary segments were cultured, in basal salts media and in media containing 1 mg/l of BAP, organogenic responses were obtained. Increased shoot proliferation and enhanced growth parameters such as increased, number of shoots, number of leaves and shoot length, was obtained in particular with BA 1 mg/l + NAA 0.1 mg/l.EnglishThesis