Sooryadas S.SARATH K.S.2020-07-232020-07-232018http://krishikosh.egranth.ac.in/handle/1/5810149712The study was conducted on fourteen adult non-pregnant female dogs of different age and body weight brought for laparoscopic birth control surgery at Teaching Veterinary Clinical Complex, Pookode. They were randomly divided into two groups of seven animals each - Group I (laparoscopic ovariectomy by intracorporeal ligation) and Group II (laparoscopic ovariectomy by haemoclip application). The study was carried out with an objective to compare the clinical efficacy of intracorporeal ligation and haemoclip application for laparoscopic ovariectomy in dogs subjected to ventral midline 3-port-laparoscopic ovariectomy. The ovary was anchored to the abdominal wall in an elevated position using suspension sutures, to facilitate ligation and resection. Laparoscopic ovariectomy by intracorporeal ligation required three ports. Two needle holders were introduced – one through the cranial and the other through the caudal instrumental ports for knot tying. Twelve centimetres long braided silk was found sufficient for applying intracorporeal flat surgeon’s knot and provided hazzle free ligation. The procedure of intracorporeal ligation was graded difficult in all the animals of Group I but provided excellent haemosatsis for ovariectomy. Hemoclip application with ventral midline three port technique was easy compared to intracorporeal ligation. The procedure of haemoclip application was graded moderately difficult in animals with considerable fat deposition around ovary and multiple clips had to be applied in the pedicle and ovarian end so as to ensure incorporation of all vessels. Haemoclip applied on ovarian pedicle with minimal fat and connective tissues were not secure. The total surgical time was 76.93±3.38 min for laparoscopic ovariectomy with intracorporeal ligation while that for hemoclip application was 61.21±2.14 min. The difference in time is attributed to difficulty in formation of knots and long learning curve, increase in number of instrument changes and surgical movements, and difficulty of intracorporeal ligation procedure compared to the procedure of haemoclip application. Intracorporeal ligation ensured more haemostatic competency inspite of its complexity. All animals returned to normal appetite within 8 to 10 h after recovery from anaesthesia. The post surgical tolerance was good and comparable between the groups with no postoperative complications. To conclude intracorporeal ligation may be preferred for ovarian pedicle haemostasis prior to ovarietomy in animals with considerable fat deposition around ovary, while hemoclip application may be limited to animals with minimal to moderate ovarian pedicle fat. Keywords: Laparoscpic ovariectomy, intracorporeal ligation, endoclip applicationennullThesis