Gupta, M. P.Renu2018-11-282018-11-282008http://krishikosh.egranth.ac.in/handle/1/5810084635There are a number of factors associated with incidence of mastitis like environmental, genetic and nutritional. The present investigations were aimed at studying the risk factors associated with mastitis in cattle and buffalo, to study tie alterations in blood coagulation, biochemical and immunological parameters in animals suffering from mastitis and to study the effect of treatment on these parameter; in animals suffering from mastitis. The prevalence of mastitis was found to be in the range of 2.38 to 3.46 %. Streptococcus spp was found to be causing majority of i ifections followed by Staphylococcus aureus and E.coli bacteria. En-ofloxacin was found to be most effective antibiotic whereas most of the strains were resistant to Penicillin. No significant difference between affected and healthy animals in their hematolotogical parameters was seen. There was no significant difference between affected and healthy animals for parameters related to blood coagualation. There was significant decline in the serum P-carotene concentration in affected animals (237 ± 40.63 1.1g/dI vs 408.08 ± 27.41 pg/d1) and the administration of Vitamin A to the mastitic animals led to a significant improvement in the condition. There was no significant difference in the level of Plasma ascorbate in mastitic (2.32 ± 0.45 mg/di) and non mastitic animals (2.50 ± 0.51 mg/di). Blood Zn concentration was found to be significantly lower in mastitic animal, whereas no difference in mean blood Cu and Co concentration was observed. There was significant rise in SCC and pH of milk in animals affected with clinical mastitis. There was found to be 5ignificant rise in LDH activity in milk from affected animals (958.96 ± 341.03 U/L vs 247.06 ± 89.47 U/L). The levels of milk LDH were observed to be good indicators of severity of mastitis and may be recommended as adjunct to diagnosis of mastitis. The level of citric acid mastitic milk was significantly lower (0.42 ± 0.29 mg/ml Ns 2.12 ± 0.35 mg/ml) and the administration of citrate to the mastitic animals helped in improving the clinical condition of the animals. Milk and serum immunoglobu dins concentration was significantly higher in mastitic animals (15.27 ± 3.58 and 30.63 ± 1.89 mg/m1 vs 6.42 ± 0.42 and 26.50 ± 1.47 mg/ml). A negative correlation between SCC and Lysozyme and Myeloperoxidase production and a positive correlation wiih Hydrogen peroxide and superoxide production was found.EnglishThesis