Shete, PBRamesh, JKarunakaran, R, et al.,TANUVAS2023-06-152023-06-152023https://krishikosh.egranth.ac.in/handle/1/5810197609TNV_ANFT_2023_23(2)319-332In the present study the nano copper and nano cobalt were synthesized by physical method using planetary ball mill. The synthesized nano particles were then characterised using Particle Size Analyzer, X-Ray Diffraction, Fourier Transform Infrared Spectroscopy, and Transmission Electron Microscope. The average particle sizes of nano copper and cobalt were found to be 84.8 and 93.5 nm, respectively. The zeta potential for nano copper and nano cobalt was found to be -18.0 mV and -13.9 mV, respectively. The particle size measured by XRD and TEM in the nano particle ranged from 1-100 nm. The XRD data showed the characteristic structure for the copper and cobalt. The FTIR analysis showed the presence of -OH and -SO4 in both copper and cobalt nanoparticles. The cell cytotoxicity study revealed dose dependent cell cytotoxicity of copper and cobalt nanoparticles. In conclusion, synthesis of nano copper and cobalt by physical method is promising field for large scale production to use for livestock feeding.EnglishVeterinary ScienceArticle