Ramji SinghMAURYA, MANOJ KUMARGopal Singh, Prashant Mishra,S. K. Sachan2018-07-312018-07-312013Population dynamics of Pseudomonas fluorescens at different moisture level on Mahua and Karanja cakeshttp://krishikosh.egranth.ac.in/handle/1/5810062754Population dynamics of Pseudomonas fluorescens at different moisture level on Mahua and Karanja cakesThe present investigations were undertaken to isolate different strains of Pseudomonas flourescens from different agro-ecological zones/crop’s rhizosphere. Total eight micro flora, resembling Pseudomonas fluorescens were isolated.Out of these, only three were confirmed as P.fluorescens,namely P.f.01,P.f.05 and P.f.07. Pseudomonas fluorescens strains P.f 07 was found most effective with highest antagonistic activity against three fungal pathogen ie. Fusarium moniliforme, Rhizoctonia solani, and Alternaria alternata. Suitability of Mahua and Karanja cakes for mass multiplication and longevity of Pseudomonas flourescens in vitro was also tested. In this study, mahua cake was found to be best substrate for supporting the population dynamics and longevity of P. flourescens in vitro. Both these cakes, supported the population of P. flourescens up to 120 days. Highest population of P. flourescens was noticed on mahua cake after 45 days of inoculation when maintained with 35% moisture. On karanja cake, highest population of P. flourescens was noticed after 45 days of inoculation when maintained with 35% moisture. Increasing concentration of extract (20%) of both the cakes, resulted in increasing the population of P. flourescens. Tomato plants grown in sterilized soil applied with P. fluorescens grown on mixture of mahua and karanja cakes resulted in highest recovery of Pseudomonas flourescens from soil after 60 days of application. Tomato plants grown in sterilized soil, applied with Pseudomonas fluorescens grown on mixture of mahua and karanja cakes, resulted in 1.5 times increase of root length, 2.0 times increase in shoot length and 6.5 times increase in fruit yields of tomato as compared to those tomato plants, which were grown in unsterilized soil, without any amendments. De-oiled cakes of mahua is comparatively better than de-oiled cakes of karanja for supporting fast multiplication of Pseudomonas fluorescens, enhancing plant growth, vigor and also fruit yieldennullThesis