SUBRAMANYAM NAIDU, K(MAJOR)VENUGOPAL NAIDU, KSURESH KUMAR, VNARAYANAN, AMBILY2018-10-082018-10-082008-02http://krishikosh.egranth.ac.in/handle/1/5810074997ABSTRACT: Eighteen bitches of different breeds presented to college and town hospital of College of Veterinary Science, Tirupati with purulent vaginal discharge were selected for this study. The diagnosis was made based on breeding history, clinical signs, abdominal palpation, radiography, ultrasonography and vaginal cytology. Smears were made to determine the vaginal cytology and vaginal discharge was subjected to antibiotic sensitivity test and sensitive antibiotic was selected for each bitch. Twelve bitches with open pyometra were divided into 2 groups consisting of 6 in each group (group I and II) and group III consisted of 6 healthy bitches kept as control. The group I bitches were treated with PGF2α (60 μg kg-1 body weight) subcutaneously for 7 days along with sensitive antibiotic and group II bitches were treated with PGF2α (150 μg kg-1 body weight) intravaginally for 7 days along with sensitive antibiotic. Group III were kept as control. In control group bitches, the mean value of TEC, Hb, PCV and TLC were 7.03 ± 0.11 x 106/cumm, 13.57 ± 0.19 g dl-1, 43.33 ± 0.5 per cent and 10.09 ± 0.44 x 103/cumm. The DLC i.e., Neutrophils, Lymphocytes, Monocytes, Eosinophils and Basophils were 69 ± 0.52, 25.5 ± 0.96, 2.83 ± 0.31, 2.17 ± 0.17 and 0 per cent, respectively. Erythrocyte indices like MCV, MCH, and MCHC were 61.72 ± 0.95 fl, 19.61 ± 0.37 pg and 32.52 ± 0.59 g dl-1, respectively. In Group I bitches the TEC, Hb, PCV and TLC before and after the treatment were 4.78 ± 0.44 x 106/cumm; 9.54 ± 0.86 g dl-1; 28.67 ± 2.62 per cent; 20.69 ± 3.30 x 103/cumm and 5.15 ± 0.4 x 106/cumm; 10.52 ± 0.81 g dl-1; 30.92 ± 2.39 per cent; 10.87 ± 0.57 x 103/cumm, respectively. Similarly the DLC i.e., Neutrophils, Lymphocytes, Monocytes, Eosinophils and Basophils were 76.17 ± 1.47; 15.83 ± 1.51; 5.0 ± 0.26; 2.17 ± 0.17; 0 and 71.5 ± 0.85; 23.83 ± 0.48; 2.5 ± 0.34; 2.0 ± 0.26; 0 per cent, respectively. Erythrocyte indices like MCV, MCH and MCHC were 60.03 ± 0.02 fl; 19.98 ± 0.02 pg; 33.28 ± 0.03 g dl-1 and 60.02 ± 0.01; 20.43 ± 0.35; 34.02 ± 0.59, respectively. While in Group II bitches, TEC, Hb, PCV and TLC before and after the treatment were 4.75 ± 0.19 x 106/cumm; 9.45 ± 0.37 g dl-1; 28.5 ± 1.12 per cent; 20.89 ± 0.94 x 103/cumm and 4.97 ± 0.20 x 106/cumm; 10.07 ± 0.46 g dl-1; 29.83 ± 1.20 per cent;10.51 ± 0.87 x 103/cumm, respectively. The DLC i.e., Neutrophils, Lymphocytes, Monocytes, Eosinophils and Basophils were 77.83 ± 0.87; 15.33 ± 0.67; 4.5 ± 0.22; 2.33 ± 0.21; 0 and 73.33 ± 0.99; 23.33 ± 0.71; 2.17 ± 0.17; 2.17 ± 0.17; 0 per cent, respectively. Erythrocyte indices like MCV, MCH and MCHC were 60.04 ± 0.01 fl ; 19.91 ± 0.02 pg; 33.15 ± 0.03 g dl-1 and 60.03 ± 0.01 fl ; 20.23 ± 0.13 pg; 33.71 ± 0.22 g dl-1, respectively. There was a significant difference in TEC, Hb, PCV, TLC and DLC between the control and pre treatment groups, pre treatment and post treatment groups but the difference was insignificant between groups. No significant difference was noticed in MCV, MCH and MCHC between control and pre treatment groups, pre treatment and post treatment groups and between groups. Biochemical parameters like BUN, Creatinine, ALT, AST, Total protein, Albumin and Globulin in healthy control bitches were 16.65 ± 0.39 mg dl-1, 1.3 ± 0.07 mg dl-1, 23.0 ± 0.37 IU/L, 27.83 ± 0.60 IU/L, 6.5 ± 0.09 mg dl-1, 3.15 ± 0.8 mg dl-1, 3.03 ± 0.21 mg dl-1, respectively. Same in Group I bitches before the treatment were 19.56 ± 1.42 mg dl-1, 1.59 ± 0.19 mg dl-1, 21.83 ± 0.56 IU/L, 28.5 ± 0.56 IU/L, 7.98 ± 0.38 mg dl-1, 2.84 ± 0.16 mg dl-1 and 5.15 ± 0.50 mg dl-1, respectively and after the treatment were 16.75 ± 0.45 mg dl-1, 1.32 ± 0.07 mg dl-1, 22.50 ± 0.76 IU/L, 27.83 ± 0.60 IU/L, 6.70 ± 0.16 mg dl-1, 3.27 ± 0.14 mg dl-1 and 3.60 ± 0.23 mg dl-1, respectively. Same biochemical parameters in Group II bitches before treatment were 19.21 ± 1.22 mg dl-1, 1.71 ± 0.18 mg dl-1 ,20.83 ± 0.78 IU/L, 29.17 ± 0.60 IU/L, 7.5 ± 0.19 mg dl-1, 3.0 ± 0.06 mg dl-1 and 4.50 ± 0.16 mg dl-1, respectively and after the treatment were 16.65 ± 0.34 mg dl-1, 1.38 ± 0.04 mg dl-1, 22.67 ± 0.88 IU/L, 28.33 ± 0.71 IU/L, 6.53 ± 0.13 mg dl-1, 3.17 ± 0.05 mg dl-1 and 3.42 ± 0.10 mg dl-1, respectively. There was a significant difference in BUN, creatinine, total protein and globulin between the control and pre treatment groups, pre treatment and post treatment groups but the difference was insignificant between groups. No significant difference was noticed in ALT, AST and albumin between control and pre treatment groups, pre treatment and post treatment groups and between groups. In the present study, vaginal cytology revealed superficial cells, intermediate cells and parabasal cells along with large number of severely degenerated neutrophils, bacteria and vacuolated endometrial cells in pyometra affected bitches. Most of the vaginal discharge samples were sensitive to Ciprofloxacin (66%) followed by Enrofloxacin (50%), Gentamicin (41.6%), Oflaxacin (25%), Kanamycin (25%), Ampicillin (25%), Pefloxacin and Amoxicillin (16%) and Cloxacillin (8.3%) and sulphonamide, tetracycline and chloramphenicol were found to be resistant. The mean progesterone concentration of healthy bitches was 11.08 ± 1.22 ng ml-1. Same in Group I and II before treatment were 10.56 ± 1.3; 10.61 ± 1.68 ng ml-1 and were significantly (P<0.05) decreased to 1.04 ± 0.39 and 0.92 ± 0.09 ng ml-1 in Group I and II, respectively indicating the luteolysis in responded bitches and 2.8 and 2.4 ng ml-1 in two non responded bitches. One bitch that did not respond even to 2nd series of injections was subjected to hysterectomy. In all the responded bitches clinical signs of pyometra had completely disappeared at the end of treatment but not in non responded cases. During the treatment trials with PGF2α no side effects were observed. It is concluded that both subcutaneous administration of PGF2α at a dose rate of 60 μg kg-1 body weight and intravaginal administration PGF2α at a dose rate of 150 μg kg-1 body weight in combination with sensitive antibiotic were found to be effective for the treatment of open type pyometra in bitches without any side effects. But the PGF2α had shown slightly better response by intravaginal route of administration than the subcutaneous route of administration in the pyometra affected bitches.ennullThesis