BALHARA, A. K.SARANGI, ARCHANA2023-11-102023-11-102019https://krishikosh.egranth.ac.in/handle/1/5810200846Evaluation of urinary metabolite dynamics pertaining to day 0 and day 42 post-insemination in healthy Murrah buffaloes (Bubalus bubalis). Metabolite analysis was performed on urine samples of pregnant, non-pregnant, nonpregnant inseminated heifers derived from three groups of artificially inseminated, thirty (n=30) healthy cyclic Murrah buffalo heifers. Urinary metabolites were analyzed in these samples corresponding to Day 0, 10, 18, 35 and day 42 of pregnancy by nuclear magnetic resonance (NMR) and the results were further validated by gas chromatography-mass spectrometry (GC-MS) analysis. Current experiments revealed and confirmed 32 metabolites in urine of both pregnant, non-pregnant buffaloes urine with global changes during early pregnancy by multivariate statistical analysis such as principal components analysis (PCA) model and heatmap. Significant increase in urinary concentration of some 2- amino-3-(1H-indol-2-yl)propanoic acid derived metabolite was observed on day 42 as compared to day 0 of pregnancy. Similarly, ethylene glycol, melatonin and fumarate appeared on specific days of early pregnancy. Contrary to this, decrease in concentration of 1-methyl-(2S)-2-amino-3-(1H-imidazol-5-yl)propanoic acid and 3-indoxysulfate were observed with progression of pregnancy from day 0 to 42. Multivariate analysis of the analysed metabolites generated distinct differential pattern with progression of pregnancy at early stage. Pathway analysis of the corresponding metabolites suggested high impact correlation with establishment and succession of pregnancy in buffaloes. Urinary metabolites measured during early pregnancy in buffaloheifers depicted close association of the metabolites with physiological processes involved in successful conception and progression of pregnancy. This study opens new avenues for use of urinary metabolites in pregnancy diagnosis in bovine especially buffaloes. Key words: Murrah buffalo; Urinary metabolites; Early pregnancy; NMR; GC-MSEnglishThesis