Ambwani, SonuRuj, Anamitra2018-08-242018-08-242017-08http://krishikosh.egranth.ac.in/handle/1/5810068856India is among those countries in the world where ancient system of medicine, such as Ayurveda, Siddha, Unani, Tribal medicine and naturopathy have been in practice for many years. Antioxidants are important substances that have the ability to protect the organism from the damage caused by the oxidative stress. From the ancient times many diseases were treated by modulating the immune system through medicinal plants. Poultry industry is highly susceptible to biotic and abiotic stresses that are incurring heavy economic losses. Immunomodulation of poultry is one measure to prevent the poultry diseases. Keeping in view of the above, present study was planned to explore immunomodulatory and antioxidative potential of Nyctanthes arbor-tristis Linn. and Moringa oleifera Lam. in chicken lymphocytes culture Extracts of these plants were prepared and evaluated for the presence of various phytoconstituents as well. The extraction yield of Nyctanthes arbor-tristis and Moringa oleifera was found to be 10.69% and 8.16%, respectively. Biochemical analyses revealed the presence of various phytoconstituents in both NAE and MOE. The total phenolics content of NAE and MOE was estimated to be 180.55mg GAE/g and 173.6mg GAE/g, respectively while total flavonoids content of the NAE and MOE was 125mg RE/g and 127.7mg RE/g, respectively. The antioxidant property of both the extracts was also evaluated by DPPH assay. Noncytotoxic doses of both the extracts were determined in the lymphocytes culture system which was found to be 300µg/ml and 250µg/ml for NAE and MOE, respectively. NAE displayed significant proliferation of 19.94% and 32.24% in T cell proliferation in case of PHA and Con A stimulated cells, respectively. There was 11.09% increase in B cell proliferation in LPS stimulated cells as compared to control. In case of MOE there was significant increase in T cells proliferation of 7.65% and 9.20% in case of PHA and Con A stimulated cells, respectively. There was 7.90% increase in B cell proliferation in case of LPS stimulated cells due to in vitro exposure of MOE. There was significant decrease in the percent Lipid peroxidation and Nitric oxide after the treatment with the plant extracts. However, GSH, SOD and Catalase levels increased in the cells treated with the extracts in comparison to control cells. Both the extracts, viz. NAE and MOE exhibited presence of antioxidants and displayed immunomodulatory effects in chicken lymphocytes culture system and thus could be used for development of immunomodulatory herbal preparations for poultry. However intensive research should be done to identify the specific phytochemicals, which are responsible for immunopotentiating activity of both plants by various biochemical and molecular biology techniques.ennullThesis