Advisor : Rathod, Dr. D. R.Authors : PIPRADE, DHANASHRI VIJAYRAO.2022-02-262022-02-262022-07-09Citation : PIPRADE, DHANASHRI VIJAYRAO. (2021). Characterization of economically important bamboo species using morphological and molecular characterization. Biotechnology Centre, Department of Agricultural Botany. Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola. M. Sc. 2021. Print. xii, 86p. (Unpublished)https://krishikosh.egranth.ac.in/handle/1/5810182963Description : Bamboo is one of the most demanding and economically important social forestry species. Bamboo has achieved worldwide attention as a resource of quick renewable biomass and a possible substitute for paper and pulpwood. Keeping in view of the vast unexploited potential of the bamboo sector, there is an urgent need to boost the cultivation of commercially required species of quantitative and qualitative characters as required by the industry sector. In the present study, bamboo is characterized based on its morphological and molecular characters and its genetic diversity has been studied. This will help to get the maximum economic benefit.Abstract : Present study entitled “Characterization of economically important bamboo species using morphological and molecular markers” was attempted at Biotechnology Centre, Dr. PDKV, Akola during 2020-21 and 2021-22. Main aim was to characterize economically important bamboo species viz., Dendrocalamus stocksii, Bamboosa balcoa, Dendrocalamus strictus, Bamboosa tulda, Bamboosa vulgaris. The morphological characterizations of these important bamboo species were attempted based on field-based observations. This was carried out by samples collected randomly from three plants of each species in field grown Bamboo Sectum, College of Agriculture, Dr. PDKV, Nagpur campus for 21 traits includes, 17 quantitative and 4 qualitative traits. B. tulda has found highest internodal distance 45.41cm, followed by D. stocksii (41.39cm) and the lowest D. strictus (30.78 cm). In case to yield, B. balcoa was found highest with 41.49 t/ha, followed by D. stocksii (35.22 t/ha), B. tulda (28.39 t/ha), B. vulgaris (17.48 t/ha) and lowest in D. strictus 12.53 t/ha, respectively. While culm height and number of culms/clumps sequentially B. vulgaris, D. strictus, B. tulda, D. stocksii and B. balcoa found 17.33m, 10.36m, 10.33m, 10.27m and 8.27m and B. tulda, D. strictus, D. stocksii, B. vulgaris and B. balcoa with 54.19, 45.22, 237.33, 26.03 and 9.51 respectively. Sp1 and sp5 were non-thorny while others are thorny natures and all spp. are economically important. Species genetic diversity was assessed using five polymorphic SSRs in all the spp. Dendrogram was constructed using Jaccard’s similarity coefficient and genotypes were grouped into three clusters based on SSR profile. A total of five SSR markers (RM-154, RM-224, RM-226, RM-338 and RM-340 were used for molecular genotyping of five important bamboo spp. Out of which, RM-224 and RM- 340 shows highest polymorphic alleles (3.0) each, followed by RM-226 (2.80), RM-154 (2.0) and least in RM-338 (1.38) Among bamboo spp., highest allelic diversity observed in D. stocksii, followed by B. tulda (13.00) and lowest in B. balcoa and vulgaris (11.00). Whereas, statistical analysis indicates that B. balcoa and B. tulda possess more genetic diversity with 47.51% and 43.85 % while B. vulgaris has lowest diverse (20.33%). Based on dendrogram, it proves that D. stocksii and D. strictus are closely related and shows highest similarity among them. While bamboo species, B. tulda and vulgaries shows closest spp. and they are genetically distance with B. balcoa. The molecular characterizing by using five SSR makers will be helpful for bamboo nursery persons for species identification and characterization of economically important bamboo species using SSR markers. The morphological and molecular markers represent similarity and distance between the bamboo spp. taken under study. The information generated will be useful for future Bamboo breeding programme.EnglishThesis