C. K. TiwariAmarjeet Thakur2023-12-202023-12-202023-09-15Amarjeet Thakur {2023} Effect of Growth Regulators on in vitro regeneration and germination behavior of Buchanania cochinchinensis (Lour.) Almeida”, M.Sc. (Horti.), Forest Biology and Tree Improvement Banda University of Agriculture and Technology Banda (U.P)https://krishikosh.egranth.ac.in/handle/1/5810203980Buchanania cochinchinensis (Lour.) M.R. Almeida is commonly known as chironji belonging to the family Anacardiaceae. It is an excellent economic fruit tree of agro-forestry and social forestry. The species is highly heterozygous and cross pollinated with gestation period of 15-20 years. It has multifarious uses as seed are edible and of high medicinal value. It attains the height up to 20 m and distributed in the states of Madhya Pradesh, Chhattisgarh, Jharkhand, Gujarat, Uttar Pradesh, Maharashtra, Orissa, Andhra Pradesh. The species is listed as a vulnerable medicinal plant in the International Union for Conservation of Nature and Natural Resources (IUCN) Red Data Book. Due to heavy unscientific extraction and lack of proper propagation protocol, there is a tremendous pressure on the natural genetic resources of the species. In view of the facts, the present study was carried out to investigate effect of various growth regulators on germination behavior of chironji seed and in vitro response of the chironji explants. The results revealed that BA 600ppm was proven best among all treatments with maximum values of germination percentage (73.67%), germination speed/rate (0.69), MDG (2.50), PV (15.00) & GV (37.50). In vitro response revealed that maximum value (24.67%) of callusing was found in internodal explants with minimum days of response of 12 days only in MS media supplemented with 2,4-D + NAA (0.9+0.6) whereas GA3 (2.0 mg/l), which was able to induce organogenesis in the cultures established through zygotic embryo with only 2% of morphogenic response in 27 days. The present study on germination behavior and in vitro studies will help the scientific community and other stakeholders to adopt seed pre-sowing treatment and in vitro approaches for the production of quality planting material of chironji for afforestation and reforestation purposes with high survival percentage on nursery and field plantation.EnglishThesis