Dr. SUGUNA RAOJEEVAN, K2021-02-062021-02-062018-07https://krishikosh.egranth.ac.in/handle/1/5810160948The present study was carried out to determine the prophylactic and therapeutic anticancerous efficacy of Zinc oxide nanoparticles (ZnO NPs) against DEN induced hepatocarcinogenesis in rats. The various groups in the study included negative control (Group I), DEN control (Group II), ZnO NPs control (Group III), ZnO NPs and DEN concurrent group (Group IV), ZnO NPs pre-treatment group (Group V) and ZnO NPs post-treatment group (Group VI). Hepatocarcinogenesis was induced by single intraperitoneal injection of DEN (100 mg/kg) on Day one followed by 0.01 % DEN in drinking water ad libitum throughout the study period. Induction of hepatocarcinogenesis was determined by the appearance of grayish white nodules on the surface of liver grossly and confirmed by microscopical examination. ZnO NPs were used at a dose rate of 25 mg/kg body weight. The effect of DEN administration and ZnO NPs treatment in different groups were assessed by hemato-biochemical parameters, oxidant and antioxidant prolife, histopathology, immunohistochemical and molecular evaluation of p53 and glutamine synthetase expression. DEN induced hepatocarcinogenesis as early as by 60th day and induced larger growths of hepatocellular carcinoma by 90th day. ZnO NPs concurrent and pre-treatment delayed the onset of DEN induced hepatocarcinogenesis and reduced the number of hepatocellular carcinoma nodules. ZnO NPs post-treatment did not reveal any effect on completely developed hepatic tumors. Based on the results, it was concluded that ZnO NPs have prophylactic effect but no therapeutic effect against DEN induced hepatocarcinogenesis.EnglishThesis