Sharma, R.Lamba, Kapil2017-08-212017-08-212006http://krishikosh.egranth.ac.in/handle/1/5810029181The present study was carried out to analyze the immunogenicity of Foot- and-Mouth Disease Virus (FMDV) antigens in sheep and lambs vaccinated with polyvalent oil adjuvanted FMD vaccine. Humoral immune response was evaluated by monitoring the serum antibody titres in the sera of vaccinated sheep and lambs on 0, 7, 14, 21, 28, 35, 45 and 60 days post vaccination (dpv) by liquid phase blocking ELISA (LPB ELISA). The FMDV specific IgG and IgM antibody titres were also estimated on different dpv by indirect double antibody sandwich ELISA. The cellular immune response was monitored by MTT based lymphoproliferation assay. Higher LPBE antibody titres were observed in sheep compared to lambs with the peak titres in both the groups obtained between 21-28 dpv. Significant LPB ELISA antibody titres were detected up to the end of study i.e. 60 dpv in sheep and lambs. Higher antibody titres were detected against FMDV serotype A followed by O and Asia1 in both the groups. The virus specific IgG antibody titres followed the same trend as observed in LPBE antibody titres. Further, IgG titres were highly correlated to LPBE antibody titres (correlation cofficient 0.86-0.90). Peak virus specific IgM titres were recorded between 7-14 dpv in both the groups and were higher in sheep compared to lambs. The lymphoproliferative responses against FMDV serotypes O, A and Asia1 in both the groups initiated as early as 7 dpv with peak response observed between 21-28 dpv followed by gradual decline in sheep but declined abruptly in lambs after attaining peak response. Significant proliferative response was maintained up to 35 dpv in sheep compared to humoral immune response, which was maintained at significant level up to 60 dpv, the day of last sampling. The lymphoproliferative responses against Con-A stimulated lymphocytes cultures in vaccinated groups were not significantly different from that of the control groups of sheep and lambs.enThesis