Dubey, AshutoshPant, Samridhi2022-12-122022-12-122022-09https://krishikosh.egranth.ac.in/handle/1/5810190562Oroxylum indicum belongs to the monotypic genus Oroxylum. It has long been used in traditional medicinal systems and is an important part of Ayurveda. In the present research the antioxidant activity and the secondary metabolites’ constitution of seed and stem bark of O. indicum in the aqueous and hexane extracts was studied. Total antioxidants (12.792±0.1 mgAAE/g, 14.961±0.04 mgAAE/g), total phenolic (136.181±0.2 mgGAE/g, 135.08±0.15 mgGAE/g), total tannin (56.999±0.8 mgTAE/g, 26.833±0.4 mgTAE/g), total flavonoid (88.911±0.41 mgQE/g, 122.925±0.30 mgQE/g) and total flavonol content (25.832±0.23 mgCE/g, 27.885±0.087 mgCE/g) was higher in aqueous extract for both seed and stem bark than hexane extract, respectively. Total antioxidants showed positive correlation with the phytochemicals. The metal ion chelating activity (IC50) (32.404±0.14 μg/mL and 30.756 ±0.27 μg/mL), DPPH scavenging (IC50) (101.396±0.8 μg/mL and 115.358±0.4 μg/mL) and reducing power (RP50) (37.115±0.02 μg/mL and 40.134±0.5 μg/mL) was also higher in the aqueous extract of seed and stem bark than the hexane extracts as expressed by their lower IC50 values respectively. All activities showed a negative correlation with the phytochemicals indicating the increase in the phytochemicals will lead to better antioxidant potential by lowering the IC50. The extracts were tested for their anti-inflammatory activity. The results were expressed as IC50 values which were 82.26±0.5 μg/mL and 79.681±0.4 μg/mL for the aqueous extract which was better than the hexane extract. Anti-bacterial activity of the extracts was tested against Xanthomonas oryzae and activity was observed only in the aqueous extract of seeds. A maximum zone of inhibition of 18 mm was observed at 5000 μg/mL. The seed proteins of O. indicum were fractionated and tested for their amylase and protease activity and inhibition. Highest amylase activity was observed in albumins and highest inhibition was observed in the prolamin. For the protease activity, albumins and globulins showed almost same protease activity but the highest inhibition was observed in globulins. The seed proteins of O. indicum seeds were partially purified using ammonium sulphate precipitation and dialysis and characterized using SDS-PAGE. The process showed 9 bands on the gel with molecular weight ranging from 97 kDa to 14.3 kDa.EnglishThesis