Sharma, AnitaSingh, Harshita2022-10-162022-10-162022-07https://krishikosh.egranth.ac.in/handle/1/5810188983Cypermethrin and Profenofos degrading bacterial isolates were recovered from the pesticide contaminated site of agricultural fields of U.S. Nagar, Uttarakhand. Recovered bacterial isolates were enriched to grow in Minimal Salt Medium in the presence of Cypermethrin and profenofos (20 to 100 ppm). Two bacterial isolates (HS2A and HS2B) were selected for the study on the basis of their ability to degrade and utilize Cypermethrin and Profenofos as a source of Carbon and phosphorus. Both the isolates tolerated 100ppm of Cypermethrin and Profenofos. Bacterial isolates were characterized on the basis of morphological, biochemical and molecular tests. HS2A was characterized as Bacillus amyloliquifaciens and HS2B with Staphylococcus gallinarum showed more than 95% s e q u e n c e homology. HS2A and HS2B biodegraded Cypermethrin and Profenofos (100ppm) in Minimal Salt Medium. After 36 h, the pattern of Cypermethrin and Profenofos degradation was 88.9% > 76.31% and 84.62% > 79.03% by HS2A and HS2B respectively. Degradation of both the pesticides in soil slurry was 61.54% > 61.21% and 54.38%, 67.01% > 66.80% and 63.86% by mixture of HS2A+HS2B and HS2A, HS2B respectively after 15 days. HS2A also decolorized toluidine blue in MSM. Pot experiment conducted on maize and wheat using unsterilized soil spiked with Cypermethrin (0.806 ml/100ml water) and Profenofos (0.714 ml/100ml water) showed best performance in consortium treatment. GC-MS analysis of intermediates of biodegraded Cypermethrin and Profenofos with bacterial isolates did not show the presence of toxic metabolites after 36 hours in Minimal Salt Medium. Bacterial isolates degraded Cypermethrin and Profenofos into Benzene, 1,3-bis(1,1-dimethylethyl)-, Dodecane, 4,6-dimethyl-, Dodecane, n-Hexadecanoic acid, Eicosane, 2,4-dimethyl- and 13-Hexyloxacyclotridec-10-en-2-one Hexane, 3,3,4- trimethyl. Soil health and microbial diversity were better in treated soil than control.EnglishThesis