Subhadra SinghMadhu2016-11-212016-11-212007http://krishikosh.egranth.ac.in/handle/1/86574A study on 39 genotypes of mungbean representing a cross-section of variability was undertaken to assess RAPD polymorphism and to estimate the matrix of Jaccard’s similarity coefficient. A total of 411 amplified products comprising 382 (92.9%) polymorphic and 29 (7.1%) monomorphics markers were observed using 30 RAPD primers. The number of bands generated per primer ranged from 7 to 20 bands with a mean of 13.7 bands per primer. The primers showed a large variation in the frequency of polymorphic bands (0.026 - 0.974) and in number of banding patterns (3 – 35). Efficiency of primers were analyzed using Discrimination power (D), Diversity index (DI), Resolving power (Rp) and Marker index (MI). All efficiency parameters except DI could identify most efficient (S-1 and S-2) and the least efficient (S-30) primers. Both discrimination power (D) and resolving power (Rp) parameters appear to be good measure of the usefulness of primers to identify cultivars. The experimental results obtained for real efficiency of the primer agreed well with that of theoretical expectation based on independent hydpothesis of primers. Genetic similarity coefficient ranged from 40.8 per cent to 90.3 per cent indicating moderate level of genetic variability among genotypes. Some of the genotypes such as K 851, Pusa Ratna, MH 98-1, Asha, MH 96-1 Muskan, Koppergaon, K 851, Pusa Baishakhi, ML 1108, PDM 262, ML 682, Pusa 9072 and ML 818 exhibited higher genetic similarity with rest of the genotypes. ML 131 was the most diverse genotype. Other genotypes namely ML1349, Pant M-4, Greengram Co-5, SML 32, UP 99-3, ML 1333, UPM-98 also exhibited higher diversity. The dendrogram constructed from Jaccard’s similarity coefficient showed three main groups. The group A consists of 26 genotypes while group B and C consist of three and two genotypes only. The mungbean genotypes evolved at PAU, Ludhiana (Punjab) and in Uttar Pradesh were comparatively more diverse among themselves than the group of genotypes from Hisar (Haryana) and IARI (Delhi). The grouping of some genotypes corresponded with their pedigree information but failed in other cases. The same was true with the geographical origin vis-à-vis grouping of genotypes. Mantel’s test between the cophenetic matrix and the original matrix (z=r=0.923) revealed a good fit of cluster analysis performed. The pattern of grouping in cluster analysis was also reflected in Principal Coordinate Analysis (PCoA) with minor differences. The three coordinate PCoA could account 73.68 per cent of total variability in RAPD markers. The high level of RAPD polymorphism observed in mungbean may be useful for variety protection and monitoring the genetic diversity.enGenotypes, Genetics, Rapd, Polymorphism, Dna, Planting, Pcr, Genetic markers, Biological phenomena, EnzymesThesis