RANA, KIRANSAPNA DEVI2022-08-202022-08-202022-08https://krishikosh.egranth.ac.in/handle/1/5810186492ABSTRACT The present investigations entitled “Seasonal incidence and management of brood diseases and greater wax moth (Galleria mellonella L.) in hive bees” was conducted during the year 2019-2021 at Department of Entomology, Dr. Y.S. Parmar University of Horticulture and Forestry, Nauni, Solan, Himachal Pradesh. European foulbrood disease incidence in Apis cerana F. colonies was maximum (17.50%) in the month of July, when temperature, relative humidity and rainfall were high. Thai sacbrood disease was recorded maximum during May (6.90%) when temperature was high and relative humidity and rainfall were low. The incidence of Tropilaelaps clareae was maximum in the month of June (7.20%) when temperature was high. In Apis mellifera L. colonies the European foulbrood disease incidence was maximum in September (37.10%) under stationary conditions when temperature was high and relative humidity and rainfall were moderate. Under migratory conditions the sacbrood disease incidence was maximum in the month of July (38.30%) when temperature was high, relative humidity was low and rainfall was maximum. Sacbrood disease incidence was maximum in May under both stationary and migratory conditions (6.60% and 5.80%) when the temperature was high and relative humidity and rainfall were moderate. Under stationary and migratory conditions incidence of V. destructor and T. clareae was observed during summer months when the temperature was high. The incidence of wax moth (larvae, pupae and adults) in A. cerana and A. mellifera colonies was maximum in the month of July under stationary and migratory conditions (7.47, 6.53) in A. mellifera and (8.97) in A. cerana colonies when the temperature and rainfall were high and relative humidity was moderate. Management studies of greater wax moth under laboratory conditions revealed that after 21 days of treatment of the combs, no infested area and reduction in weight of the combs (A. cerana and A. mellifera) was recorded in deep freezing at -8℃ to -10 ℃ followed by Bt (3.95%, 3.99%), NSKE, sulphur fumigation and Neem oil treatment. Maximum larval and pupal mortality was also recorded in deep freezing followed by Bt and minimum adult emergence was recorded in Bt (12.50%). Under field conditions placing wax moth trap and delta trap fitted with A. dorsata comb in front of the hives showed that maximum adult moths were trapped in wax moth trap and delta trap with A. dorsata comb. Out of three Bt concentrations (3, 6 and 9g/l) the highest larval mortality with less comb damage was recorded in Bt 9g/l. The IPM practices comprising cultural (periodic cleaning), Mechanical (delta trap installation) and chemical (lime sulphur application) when integrated helped in total protection of both bee colonies with no infestation in the colonies. For the non-chemical treatment of the brood diseases (sacbrood and European foulbrood) in A. mellifera and A. cerana colonies, different plant and fungal extracts were used and maximum reduction in percentage of diseased larvae in treated colonies over the diseased control were recorded in Ganoderma lucidum (3ml/250ml of sugar solution) in sacbrood and European foulbrood infected colonies.EnglishThesis