Dr. P. H. VataliyaPREETI DWIVEDI2017-09-182017-09-182007http://krishikosh.egranth.ac.in/handle/1/5810031338Prolactin is a polypeptide hormone localized on chromosome 23 and is responsible not only for triggering lactation but also for mammary gland growth and lactogenesis in almost all species. A silent A → G mutation in the codon for amino acid 103 in exon 3 of bovine PRL gene gives rise to polymorphic Rsa I site and PRL – Rsa I locus had a significant effect on milk yield and milk fat % in dairy cattle. Therefore, the present study was undertaken with the objectives to detect polymorphism in the prolactin gene (exon 3) using PCR-RFLP and PCR-SSCP in Kankrej cattle and Surti goat, both utilized as dual purpose breedsenThesis