. CHANDRASHEKAR, S. CAMAYYA MATH, SIDDALINGAYYA2019-02-202019-02-202007-09-19TH-8923http://krishikosh.egranth.ac.in/handle/1/5810096197Groundnut transgenics {Arachis hypogaea L.) cv. TMV-2 expressing glucanase, NPRl/Defensin and combination of both the genes were developed using an Agrobacterium tumefaciens (LBA 4404/pKVD 4 for glucanase and GV 2260/pCAMBIA 2300 for NPRl/Defensin) mediated in planta transformation, a tissue culture-independent method. PGR analysis of the genomic DNA isolated from T2 and T3 generation transgenics showed the integration of transgenes in the genome of the groundnut plants. The expression of the heterologous glucanase, NPRl/Defensin and combination of both the genes (NPRl/Defensin in glucanase transformants) driven by CaMV 35S promoter led to a high level of activity in some of the transgenic plants. Pot culture experiments indicated increased ability of these plants to resist tikka disease. These results suggest that a heterologous glucanase, NPRl/Defensin and combination of both the genes (NPRl/Defensin + glucanase) were functional in groundnut and expressed in healthy plants. The genomic Southern analysis showed the integration and stability of transgenes in the groundnut plants. The segregation pattern revealed that the transgenes followed the Mendalian pattern of inheritance of monohybrid ratio 3:1. Based on in-vitro and in-vivo bioassay against Cercospora spp. the resistant plants were selected. The transgenics expressing glucanase were three plants, where transgenics expressing NPRl/Defensin and NPRl/Defensin+glucanase were four and nine plants respectivelyennullThesis