Krishna Reddy, M.ASHWATHAPPA, K. V.2017-07-082017-07-082014-07-10Th-10952http://krishikosh.egranth.ac.in/handle/1/5810024690Cucumber Mosaic Virus (CMV), which is widely distributed having a wide host range, affects seriously both Hot and Bell peppers in Karnataka. Survey during 2013-14 in Southern Karnataka has indicated the incidence of CMV from 33.33 to 50.00 per cent in different locations based on visual observations; where as analysis of symptomatic and non-symptomatic samples by ELISA showed 24.00 to 40.00 per cent. Two isolates viz., CMV-KO and CMV-R inoculated to 27 plant species, of which seven species showed CMV infection. Biological and molecular variability was noticed in different geographical locations. The complete genome sequence of CMV-KO isolate was determined by sequencing the PCR amplified fragments. The genome of CMV-KO comprises of RNA1 with 3358nt, containing a single ORF encoding 1a protein, which is required for viral replication with methyltransferase and helicase motifs. The RNA2 of 3045nt long contains two ORFs i.e. ORF2a and ORF2b. The ORF2a codes for replication associated protein and ORF2b overlapping with 2a coding silencing suppressor protein. The RNA3 of 2220nt long also contains two ORFs i.e. the ORF at the 5’, which encodes the movement protein (3a); and the ORF at 3’, which codes for coat protein (3b); with an intergenic region of 299nt. Sequence analysis revealed that 91-94 per cent homology with Ia subgroup isolates and less than 75 per cent homology with subgroup II isolates. Out of 50 Capsicum genotypes screened by mechanical inoculation, 25 genotypes exhibited immune reaction and two genotypes resistant reaction, which was confirmed by ELISAennullThesis