Dr. P. H. VataliyaNILAM BHARATSINH JHALA2017-08-012017-08-012008http://krishikosh.egranth.ac.in/handle/1/5810026875Leptin is a 16-kDa protein synthesized by adipose tissue and is involved in regulation of feed intake, energy balance, fertility, and immune functions. Leptin binds to a receptor mainly localized on neuropeptide Y-neurons. The bovine leptin gene is organized into three exons separated by two introns, of which only 2 exons (exon-2 and 3) are translated into proteins. Variation in the exonic region of a gene may lead to changes in amino acids which alter the expressed protein. Therefore, the present study was undertaken to detect polymorphism at respective restriction sites in Leptin gene exon-3 by PCR-RFLP, SSCP, and sequencing in Gir cattle and Mehsana buffalo breedsenThesis