VivekanandOm Prakash2019-02-042019-02-042018-08http://krishikosh.egranth.ac.in/handle/1/5810093548Citrus species are the member of Rutaceae family which includes flowering trees and shrubs like oranges, lemons, grape fruit and limes. They posses several health promoting properties due to the presence of some bioactive secondary metabolites like Polyphenols, having several nutritional and antioxidants potential. The present study was undertaken with the objective of, biochemical profiling, genetic profiling, antibacterial screening and herbicidal activity of methanolic extract of peel of four different citrus species (Citrus jambhiri, Citrus lemon, Citrus aurentifolia, and Citrus sinensis) collected from 4 different districts of Uttarakhand. GC-MS analysis of extracts showed significant variation in their phytoconsitituents. Maximum 92.99 % compounds identified in Citrus jambhiri (Pithoragarh), 78.80 % in Citrus jambhiri (Almora), 84.54 % in Citrus sinensis (Nainital) and 93.37 % in Citrus aurantifolia (Rudraprayag). Total phenols, flavonoides and tannin are ranging from 46.9±1.11 to 20.10±0.20 mg/g GAE, 58.04±2.49 to 19.21±04 mg/g CNE, and 30.06±2 to 4.23±1.45 mg/g TAE respectively. A significant antioxidant potential was shown in all species in a dose dependent manner. A significant antibacterial activity of citrus sp. was evaluated against various gram positive and gram negative bacteria. The order of magnitude of macro and micro essential elements is Na>Fe> Ca> Zn> Ni> Cu> Mn> Co. Metal complexes of Rutin and bioconjugate of citrus peel extract showed moderate to highs herbicidal activity against Lathyrus aphaca and Mellitus alba. SSR primer technique was performed to study genetic diversity among Citrus accessions. Metal complexes of Rutin and bioconjugate of Citrus peel extract also exhibit strong antioxidant and herbicidal activity.ennullThesis