Galdhar, C. N.Alone Pallavi Dipakrao2024-10-302024-10-302024-04-10https://krishikosh.egranth.ac.in/handle/1/5810215981The present study was undertaken to establish data about canine TSH and its association with Total triiodothyronine (TT3), Total thyroxine (TT4), and Free thyroxine (fT4) in healthy dogs; and to study alteration in canine TSH, Total triiodothyronine (TT3), Total thyroxine (TT4) and Free thyroxine (fT4) in suspected cases of thyroid dysfunction, if any. The present study enrolled a total of 77 dogs, with 67 healthy and 10 hypothyroid. The canine-specific TSH ELISA kit was used to measure the serum concentration of cTSH, while a RIA kit was used to measure the serum concentration of Total triiodothyronine, Total thyroxine, and Free thyroxine. The mean concentration of Total triiodothyronine (TT3), Total thyroxine (TT4), Free thyroxine (fT4), and Thyroid stimulating hormone (cTSH) in healthy dogs were 0.85 ± 0.04 nmol/l, 20.81 ± 0.78 nmol/l, 12.59 ± 0.35 pmol/l and 2.03 ± 0.01 μIU/ml respectively. In hypothyroid dogs, the mean concentration of Total triiodothyronine (TT3), Total thyroxine (TT4), Free thyroxine (fT4), and Thyroid stimulating hormone (cTSH) was 0.93 ± 0.07 nmol/l, 9.01 ± 0.69 nmol/l, 5.87 ± 0.82 pmol/l and 2.16 ± 0.03 μIU/ml respectively. The study reported significant changes in the concentration of TT4, fT4, and cTSH between healthy and hypothyroid dogs. In the case of healthy dogs, the reference range (25th to 75th percentile) of cTSH was 1.96 -2.11 μIU/ml while in the case of hypothyroid dogs, the reference range (25th to 75th percentile) of cTSH was 2.09-2.22. Out of 10 hypothyroid dogs, 70% had a reference range above the healthy range, while 30% had a reference range equal to the healthy range. The sensitivity (%) and specificity (%) of the measurement of cTSH were 63 % and 72 % respectively. The study revealed discrepancies in clinical findings, hematobiochemical results, and thyroid profiles in both healthy and hypothyroid dogs.EnglishThesis