Kher, H. N.Gandhi, Sandip S.2018-05-252018-05-251992http://krishikosh.egranth.ac.in/handle/1/5810047490The present study was undertaken in order to screen the faecal samples from diarrhoeic calves and to compare with known rota virus isolates with respect to genetic difference and relative sensitivity of the tests viz., PAGE, Rotalex latex agglutination tests and EM. Faecal samples of 35 diarrhoeic calves were first examined by PAGE. Of these, only one sample from ten-day-old cow-calf was found positive for rota virus by PAGE, Rotalex' latex agglutination test and PAGE were found equally sensitive in detection of rota virus from faecal samples of diarriipeic calves. In the EM study, typical shape of rota virus was observed. The size of individual virus particles was 62 nm. Virus was successfully isolated in MDBK cell - line and produced demonstrable changes after two blind passages. Typical CPE characterized by extensive vacuolation, roundening of the cells and detachment of the cells from the glass surface was observed in MDBK cell-line . The stained coverslips cultures examined at regular intervals showed small vacuolations, crescent shaped and spindle shaped cells. Some of the infected cells exhibited intracytoplasmic inclusion bodies. Crystalline trypsin was used for treating the faecal sample and in the maintenance medium for better recovery of virus. In the electropherogram study of tissue culture adapted isolates of rota virus, it was observed that Indian isolates resembled to UK strain.enVERTINARY MICROBIOLOGYA STUDYThesis