Sarawat, ShwetaRawat, Sumit2019-07-172019-07-172019-05http://krishikosh.egranth.ac.in/handle/1/5810115052The present study was undertaken to assess the hydrogen production potential of rice straw, vegetable agro-wastes and their combination employing Klebsiella pneumoniae, Escherichia coli and their mixed inoculum under anaerobic conditions. All the pretreated substrates i.e. rice straw, vegetable waste, and rice straw+vegetable waste yielded higher hydrogen gas as compared to their untreated substrates. In general, the maximum hydrogen production was obtained at pH 6.0, OD 0.5 and temperature 37° C in case of each treatment. Among all substrates, rice straw yielded maximum hydrogen gas throughout the experiment. The pretreated rice straw yielded maximum hydrogen gas i.e. 65.6 ml / 100 g VS using mixed culture followed by 64.2 ml / 100 g VS using K. pneumoniae and 56.8 ml / 100 g VS using E. coli at pH 6.0 and OD 0.5. In case of mixed culture i.e. K. pneumoniae+ E. coli the hydrogen gas production was recorded maximum i.e. 240.46 ml / kg rice straw followed by K. pneumoniae (220.1 ml / kg rice straw) and E. coli (160.2 ml / kg rice straw) at pH 6.0 and OD 0.5. The ability to produce hydrogen by mixed culture was 10% higher than K. pneumoniae and 33% higher than E. coli. The results revealed that the pretreated rice straw in combination of K. pneumoniae and E. coli can be used to produce green renewable energy for the sake of agrowastes and environmental management.ennullThesis