Sangha, G.K.Mokshi2017-06-032017-06-032015http://krishikosh.egranth.ac.in/handle/1/5810015848Dimethoate, a systemic organophosphate pesticide, was used in the present study to investigate toxic effects on blood, liver, kidney, brain and reproductive organs of female albino rats at three sub-chronic dose levels of 1/20 th , 1/40 th and 1/80 th of LD50 for 30 days and the ameliorative potential of 20% wheat germ supplementation as a source of tocopherol was evaluated against the dimethoate treatment. Feed intake and water intake was non significant in all treatment groups. Estrous cyclicity was significantly altered with 1/20 th and 1/40 th of LD 50dimethoate. After 30 days of treatment, blood plasma collected and various organs excised and weighed. Amount of protein, urea, creatinine, enzymes alanine and aspartate aminotransferase (ALT and AST), acid and alkaline phosphatase (ACP and AKP), acetylcholinestrase (AChE) and ATPases and activity levels of different oxidative stress parameters viz: catalase (CAT), superoxide dismutase (SOD), glutathione-S-transferase (GST), glutathione reductase (GR), glutathione peroxidase (GPx) and lipid peroxidation (LPO) were differentially altered in blood, liver, kidney, brain and ovary of dimethoate treated rats. Liver histological observations depicted infiltration, sinusoidal dilations and degenerated cytoplasm at highest dimethoate treatment. The kidney histoarchitecture with dimethoate intoxication also revealed shrunken glomerulus, glomerular atrophy and disturbed tubular epithelium as compared to control. Increased follicular atresia, was observed with the increasing dose levels of dimethoate. The preconceptional exposures of dimethoate in female rats also affected the eproductive performance and developmental parameters in the offsprings. 20% wheat germ upplementation improves the histoarchitecture of liver, kidney and ovary and restored the activity of biomolecules and enzymes in all dimethoate treated rats. In conclusion it can be inferred that dimethoate has the potential to alter the biochemical constituents including OS enzymes in female rats and 20% wheat germ supplementation has protective effect against dimethoate toxicity in female albino rats.enbiological phenomena, enzymes, pesticides, inorganic compounds, organic compounds, vitamins, toxicity, wheats, irrigation, processed plant productsThesis