Saseendranath, M RIndu, SKAU2019-05-072019-05-071997http://krishikosh.egranth.ac.in/handle/1/5810102231PGThe seroprevalence of leptospirosis in one hundred and fifty dogs from different parts of Thrissur including 138 dogs presented to the Hospital and twelve stray dogs were studied by microscopic agglutination test. Among the 150 dogs, 49 (32.67 per cent) were seropositive to Leptospira serovars pomona (53.06 per cent), canicola (32.65 per cent) and icterohaemorrhagiae (14.28 per cent) 83.33 per cent of the stray dogs were seroreacative with agglutinins to L. serovars pomona (90 per cent) and L. canicola (10 per cent) . Seroprevalence recorded was higher age wise among young dogs, upto three years of age, among the male dogs and among non-descript and German Shepherd dogs. Monthwise prevalence was higher during June, May and December. DFM examination were carried out on blood samples from 23 suspicious cases of leptospirosis and 12 stray dogs, and on urine sample from eight suspected cases. Leptospiraemia was detected in two clinical cases (C 261 and C 11556) and from two stray dogs (S-6 and S-10) Urine sampies were negative for leptospires or DFM. Culture and isolation were tried with blood sample from 23 suspected cases of leptospirosis and from 12 stray dogs, urine sample from eight suspected cases of leptospirosis and kidney tissue from six sacrificed stray dogs. Of these, blood sample from stray dog 8-6 showed evidence of growth in the medium, but leptospires could not be isolated. The culture medium with kidney tissue from stray dog 8-6 revealed the presence/growth of leptospires on DFM examination. But isolation of leptospires from amongst the contaminants was not possible on further subculture, millipore filtration or guinea pig inoculation. Haematological findings in the seropositive dogs were monocytosis which was highly significant, non- significant i~crease in the mean values of E8R, total leucocyte count and absolute neutrophil count. A slight increase in the mean PCV, absolute lymphocyte and eosinophil count were recorded. Mean haemoglobin level was slightly lower in the seropositive dogs, compared to the seronegative dogs. On biochemical analysis, significant increase (PsO.05) in creatinine level was recorded in the seropositive dogs. There was increase in the mean total bilirubin, albumin and albumin-globulin ratio in the seropositive dogs, but it was non-significant. Mean serum total protein, globulin, serum ALT values were lower in the seropositive group compared to those of the seronegative group.ennullThesis